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. 2024 Jan 27;14(2):189. doi: 10.3390/life14020189

Figure 2.

Figure 2

Apoptotic properties of extracts of non-symbiotic and symbiotic L. japonicus plants on HEK-293 cells. A Western blot analysis of procaspace-3 and bcl2 after the application of shoot and root extracts to human cell cultures at a concentration of 20 μg/mL and 48 h incubation. (a) Protein levels after the application of extracts from AMF-inoculated plants or non-inoculated plants grown at 24 °C, (b) protein levels after the application of extracts from Rhizobium-inoculated plants or non-inoculated plants grown at 21 °C. Data are expressed as ratios of the levels of the apoptosis-related proteins to the respective levels of β-actin. The relative band density of control DMSO-treated cells was set as 1. DMSO: control DMSO-only treatment; C1: extracts from non-inoculated plants grown at 21 °C; C2: extracts from non-inoculated plants grown at 24–25 °C; AMF: extracts from AMF-inoculated plants (Greek isolate); AMF-D: extracts from AMF-inoculated plants (DAOM strain); R: extracts from Rhizobium-inoculated plants.