Table 2.
Accumulation of metabolites of L. japonicus ethyl acetate extracts in roots vs. shoots. Data are presented as ratios of metabolite accumulation in roots to metabolite accumulation in shoots. In bold: two-fold or greater change; In red: higher accumulation in roots than shoots (five-fold or greater); In blue: higher accumulation in shoots than roots (five-fold or greater).
| Metabolites | Root/Shoot Ratios | ||||
|---|---|---|---|---|---|
| * Control | AMF | AMF-D | R | R + D | |
| Phenolics | |||||
| vanillic acid | 10.4 | 14.4 | 2.1 | 27.2 | 17.0 |
| apigenin 7-glucoside | 0.3 | 1.8 | 2.5 | 0.0 | 0.2 |
| cyanidin3-rytinoside | 0.1 | 0.5 | 0.9 | 0.0 | 0.6 |
| hydroxycaffeic acid | 0.3 | 0.0 | 0.1 | 0.9 | 1.3 |
| isoorientin | 0.5 | 0.8 | 0.9 | 0.2 | 0.6 |
| isorhoifolin | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
| lariciresinol | 0.7 | 1.6 | 2.7 | 1.9 | 3.4 |
| luteolin-5-glucoside | 0.5 | 0.8 | 0.9 | 0.2 | 0.6 |
| quercetin-3-rutinoside | Shoot sp. | Shoot sp. | Shoot sp. | Shoot sp. | Shoot sp. |
| gamma tocopherol | 20.0 | 123.1 | 5.0 | 6.4 | 42.0 |
| m-coumaric acid | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
| maachiain | 1.0 | 1.8 | 0.5 | 1.3 | 26.1 |
| glycinol | 1.1 | 0.9 | 0.9 | 1.0 | 1.0 |
| phaseollin | 94.4 | 14.3 | 10.1 | 149.3 | |
| vestitol | 0.0 | 0.0 | 0.0 | 0.5 | 0.1 |
| sophorol | 0.7 | 1.5 | 0.1 | 0.5 | 4.0 |
| (6aR,11aR)-3,9-Dihydroxypterocarpan | 1.1 | 4.5 | 0.7 | 10.9 | 10.2 |
| 2′-Hydroxyformononetin | 1.0 | 1.8 | 0.5 | 1.3 | 26.1 |
| astragalin | 0.5 | 0.8 | 0.9 | 0.2 | 0.6 |
| chalconaringenin | 1.1 | 0.9 | 0.9 | 1.0 | 1.0 |
| delphinidin | 0.3 | 1.2 | 0.4 | 0.2 | 0.1 |
| eugenol | 49.0 | 0.8 | 0.8 | 1.3 | 1.0 |
| formononetin | 0.2 | 1.6 | 0.0 | 2.5 | 0.9 |
| genistein | 0.3 | 0.6 | 0.9 | 2.4 | 1.5 |
| genistin | 0.2 | 0.6 | 0.8 | 56.9 | 0.2 |
| homogentistic acid | 10.4 | 14.4 | 2.1 | 27.2 | 17.0 |
| leucopelargonidin | 4.7 | 0.9 | 1.1 | 1.1 | 1.1 |
| lotaustralin | 0.1 | 0.1 | 0.0 | 0.6 | 0.4 |
| pelargonidin | 0.3 | 0.6 | 0.9 | 2.4 | 0.1 |
| phenylalcetaldehyde | 0.0 | 0.0 | 0.0 | 0.0 | 0.1 |
| sinapaldehyde | 0.8 | 1.0 | 1.0 | 2.0 | 0.6 |
| vestitone | 0.8 | 0.9 | 1.0 | 1.3 | 1.1 |
| coniferyl acetate | 1.1 | 0.9 | 1.0 | 1.1 | 1.1 |
| astragalin | 0.5 | 0.8 | 0.9 | 0.2 | 0.6 |
| caffeic acid hexoside | 0.3 | 0.0 | 0.1 | 0.9 | 1.3 |
| coniferaldehyde | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
| diosmetin | 0.7 | 1.5 | 0.1 | 0.5 | 4.0 |
| eriodictyol | - | - | - | 5.4 | - |
| N-containing compounts | |||||
| caffeine | 0.5 | 0.3 | 0.3 | 0.8 | 0.8 |
| cinchonidine | 0.9 | 1.0 | 0.3 | 0.1 | 1.0 |
| theobromine | 0.6 | 0.1 | 1.2 | 2.2 | 1.6 |
| portulacaxanthin | 0.5 | 0.1 | 0.1 | 0.1 | 1.2 |
| Terpenes | |||||
| oleanolic acid | 1.9 | 0.6 | 0.0 | 1.4 | 1.6 |
| ursolic acid | 1.9 | 0.6 | 0.0 | 1.4 | 1.6 |
| menthone | 0.6 | 1.1 | 0.6 | 0.3 | 0.4 |
| Sterols | |||||
| desmosterol | 1.3 | 1.4 | 1.1 | 1.2 | 1.0 |
| Fatty acids | |||||
| myristoleic | 0.9 | 1.5 | 1.2 | 2.0 | 3.9 |
| octadecanoic acid | 0.9 | 1.6 | 1.0 | 1.0 | 2.3 |
| oleic acid | 1.0 | 1.1 | 2.0 | 3.5 | 3.2 |
| palmitoleic acid | 0.9 | 1.4 | 1.3 | 1.9 | 4.4 |
| pentadecanoic acid | 1.3 | 1.4 | 1.1 | 2.3 | 3.2 |
| glycerol tributanoate | 0.5 | 1.2 | 2.8 | 1.7 | 3.1 |
* Control: extracts from non-inoculated plants; AMF: extracts from AMF-inoculated plants (Greek isolate); AMF-D: extracts from AMF-inoculated plants (DAOM strain); R: extracts from Rhizobium-inoculated plants; R + D: extracts from Rhizobium–AMF double-inoculated plants.