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. 2024 Feb 4;10(2):130. doi: 10.3390/jof10020130

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Figure A7 — Apoptosis assays using Annexin V and propidium iodide. Cells were incubated with moderate/low (36 μg/mL) and high (54 μg/mL) doses of Cunninghamella elegans FALS and then trypsinized. The apoptotic/necrotic and ferroptotic portions (of detached cells) were analyzed with flow cytometry. Alive cells are shown as Annexin V-/PI-; cells in early apoptosis are represented by Annexin V+/PI-; cells in late apoptosis appear as Annexin-V+/PI+; and necrotic or ferroptotic cells are Annexin V-/PI+. (A) Alive PC-3 cells were used as a control sample, with a stable rate of apoptosis of about 0.5%. (B–D) The effects of the low FALS dose were assessed at 12, 24, and 48 h, and loss of cell viability down to 84% was documented. (F–H) The effects of the high dose were assessed and found to decrease cell viability by 21%. (E) The fattened cells were found to have a great portion of non-viable cells, despite the constant presence of FALS.

Figure A7 — Apoptosis assays using Annexin V and propidium iodide. Cells were incubated with moderate/low (36 μg/mL) and high (54 μg/mL) doses of Cunninghamella elegans FALS and then trypsinized. The apoptotic/necrotic and ferroptotic portions (of detached cells) were analyzed with flow cytometry. Alive cells are shown as Annexin V-/PI-; cells in early apoptosis are represented by Annexin V+/PI-; cells in late apoptosis appear as Annexin-V+/PI+; and necrotic or ferroptotic cells are Annexin V-/PI+. (A) Alive PC-3 cells were used as a control sample, with a stable rate of apoptosis of about 0.5%. (B–D) The effects of the low FALS dose were assessed at 12, 24, and 48 h, and loss of cell viability down to 84% was documented. (F–H) The effects of the high dose were assessed and found to decrease cell viability by 21%. (E) The fattened cells were found to have a great portion of non-viable cells, despite the constant presence of FALS.