Table A1.

Summarization of values assigned in the central composite design (CCD) experimental set-up involving three variables with one response (weight of FFAs in g) in the saponification step for fatty acid salts production of the C. elegans lipids.

	Variables			Response
Run	A: Time (min)	B: Hexane (mL)	C: Salt (g)	Weight of FFAs (g)
1	82.5	18.0	2.0	0.6824
2	60.0	21.0	1.0	0.7693
3	82.5	13.0	2.0	0.5876
4	82.5	18.0	2.0	0.6948
5	105.0	21.0	3.0	0.7414
6	82.5	18.0	2.0	0.6976
7	120.3	18.0	2.0	0.7906
8	82.5	23.0	2.0	0.6926
9	105.0	15.0	1.0	0.7518
10	82.5	18.0	2.0	0.7583
11	44.7	18.0	2.0	0.8732
12	82.5	18.0	0.3	0.7632
13	82.5	18.0	2.0	0.7364
14	60.0	15.0	3.0	0.6810
15	60.0	15.0	1.0	0.7475
16	105.0	21.0	1.0	0.7290
17	82.5	18.0	3.7	0.6722
18	82.5	18.0	2.0	0.6739
19	105.0	15.0	3.0	0.7558
20	60.0	21.0	3.0	0.7429