FIG. 2.

Morphology and growth characteristics of stable PC12 transfectants expressing p42 ERK2. (A to C) Mock-transfected PC12 cells; (D to F) PC12 cells transfected with p42YF185, the mutant ERK2 in which tyrosine 185 was mutated to phenylalanine; (G to I) PC12 cells transfected with p42wt. (A, D, and G) Unstimulated PC12 cells; (B, C, E, F, H, and I) PC12 cells stimulated with NGF for 4 days; (C, F, and I) PC12 cells labeled with rhodamine phalloidin to stain F-actin. Arrowheads indicate processes where F-actin is concentrated in mock-transfected cells and p42wt-transfected PC12 cells. A honeycomb pattern indicates that actin extends uniformly around the periphery of cells transfected with p42YF185. Bars, 24 μm.