FIG. 3.
Assay for basal MAP kinase (ERK) activity in PC12 transfectants. (A) Basal ERK activity is altered in PC12 cells transfected with ERK cDNAs. (Upper panel) Basal activity of total ERK in transfected PC12 cells as measured by immunoprecipitation and incubation with MBP and [γ-32P]ATP. (Lower panel) ERK immunoblot showing equal amounts of immunoprecipitated ERK. (B) Quantification of ERK activity in p42YF185 cells, mock-transfected cells, and p42wt cells. The values are means ± standard errors of the means from three experiments.