FIG. 1.

Factor-dependent myeloid FDCW2 and FDCW2ER cells as a null background for studies of GATA-1 function. In FDCW2 and derived FDCW2ER cells, expression of Mac 1, Fli 1, Epo receptor, GATA-1, and β^maj-globin transcripts was assayed by Northern blotting of polyadenylated RNA. As positive controls for erythroid transcripts, polyadenylated RNAs from SKT6, FMEL, and BaF/3 cells were coanalyzed. Hybridizations were performed sequentially with a single blot, and equivalence in loading was assessed by hybridization to a GAPDH probe.