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GATA-1 expression in FDCW2ER-pXG1 cells induces c-mpl gene expression. Polyadenylated RNA was isolated from parental FDCW2 and FDCW2ER cells and from polyclonal FDCW2ER-pXG1 cells, a derived clonal subline, c.12, c.12 cells transfected with a pCG1 expression vector (to reinforce GATA-1 expression), and this c.12 subline transfected with a pCEKLF expression vector. The blots were hybridized to 32P-labeled cDNA probes for murine c-mpl and GAPDH.
