FIG. 7.

Activation of erythroid gene expression in FDCW2ER-pXG1 cells. In polyclonal FDCW2ER-pXG1 cells (lane 3), the derived clonal subline c.12 (lane 4), c.12 cells transfected with a pCG1 expression vector (to reinforce GATA-1 expression) (lane 5), and this c.12 subline transfected with a pCEKLF expression vector (lane 6), levels of expression of the following transcripts were assayed by Northern blotting of polyadenylated RNA: GATA-1, EKLF, β^maj-globin, FOG, Mac 1, ets-1, Epo receptor, and GAPDH. Polyadenylated RNA from parental FDCW2 and FDCW2ER cells served as negative controls (lanes 1 and 2), while RNA from SKT6 cells served as a positive control (lane 7). In hybridizations, a single blot was probed sequentially and equivalence in loading was confirmed by hybridization to a GAPDH probe.