FIG. 8.

Levels of GATA-1 and EKLF transcript expression among FDCW2ER-pXG1 sublines. Clonal sublines of FDCW2ER-pXG1 cells were isolated by dilution (clones c.1, c.8, c.9, c.11, c.12, c.7, and c.6 representative of 12 clones analyzed [Table 2]) and levels of GATA-1 and EKLF transcript expression were assayed by Northern blotting of total RNA. FDCW2 and FDCW2ER cells were analyzed as negative controls, while erythroleukemic SKT6 cells were used as a positive control for erythroid gene expression. Hybridizations were performed sequentially with a single blot, and equivalence in loading was confirmed by hybridization to a GAPDH probe.