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. 2024 Jan 8;43(9):668–681. doi: 10.1038/s41388-023-02924-y

Fig. 4. Regulatory mechanism of TF expression.

Fig. 4

A H2122, H1373, H1792 and HCC44 cells were treated with MTI-31 as indicated for 48 h and immunoblotted. B The same four cell lines were treated with 5 μM MTI-31 or 1 μM MRTX849 for 48 h and subjected to qRT–PCR analysis for TF mRNA levels. C, D Cells of H2122 and H1373 (C), H1792 and HCC44 (D) were treated with 5 μM MTI-31 alone or in combination with 10 nM PS-341 or 10 μM CQ for 48 h, subjected to immunoblotting. E sh-NT, sh-TF1, sh-RIC, sh-RAP HCC44 cells were pre-induced with doxycycline for TF depletion then subjected to immunoblotting as indicated. F Cells as in (E) were treated with titrated doses of MRTX849 (left) or AMG510 (right) for 72 h. Dose response curves are shown. G TF mRNA in doxycycline-preinduced sh-RAP and sh-RIC HCC44 cells. H Doxycycline-preinduced sh-RAP and sh-RIC HCC44 cells alone or in combination with 10 nM PS-341 or 10 μM CQ for 48 h, subjected to immunoblotting.