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. 2024 Feb 23;14:4469. doi: 10.1038/s41598-024-54956-3

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Kidney DPT cell have a distinct basal metabolic profile compared to CD4, CD8 and DNT cells. (A–G) A total of 1 × 10⁶ KMNCs were stained with Zombie NIR followed by surface markers (CD45, TCR, CD4, CD8) and intracellular markers (GLUT1, HKII, CPT1a, pS6, VDAC1, TOMM20 and H3K27me3) to assess DPT cell metabolism (n = 10). DPT cells had a distinct metabolic profile that was intermediate between CD4 and CD8 T cells. Data are expressed as mean ± sem and compared by non-parametric Kruskal–Wallis test followed by Dunn’s post-hoc analysis. * = P ≤ 0.05, ** = P ≤ 0.01.