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. 2024 Feb 23;14:4469. doi: 10.1038/s41598-024-54956-3

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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scRNA-Seq analysis of DPT cells at baseline and 24 h post-IR kidneys of WT mice. CD45+ cells were flow sorted from pooled KMNCs (n = 5/group) and 10,000 cells targeted for 10X Chromium-based encapsulation. (A) UMAP showing major immune cell populations identified in the scRNA-seq data. (B) UMAP showing different T cell populations and the presence of naive DPT cells in WT kidneys. (C and D) Plots showing DPT cell proportions in control and post-IR kidneys. (E–G) Volcano plots showing top differentially expressed genes (DEGs) in naïve DPT cells compared to effector CD4, CD8 and DNT cells, respectively. (H–J) Volcano plots showing top differentially expressed genes (DEGs) in naïve DPT cells compared to naive CD4, CD8 and DNT cells. (K, L) Gene set enrichment analysis (GSEA) of differentially expressed genes (DEGs) in showing increased enrichment of TNFα signaling in DPT cells compared to CD4 and CD8 cells and (M) increased OXPHOS compared to DNT cells.