Fig. 4.

Inactivation of Piezo1 in chondrocytes impairs trabecular bone mass and limits OA development after ACLT. a Representative von Kossa-stained sections of undecalcified vertebral bodies of control, Piezo1^Col2a1cre, and Piezo2^Col2a1Cre mice. Scale bar: 500 µm. b Histomorphometric quantification of trabecular bone volume (BV/TV), trabecular thickness (Tb. Th), and trabecular number (Tb. N) (n ≥ 8). c Representative µCT images of knee joints from non-operated and ACLT control, Piezo1^Col2a1cre, and Piezo2^Col2a1Cre mice showing reduced subchondral bone volume in non-operated and ACLT Piezo1^Col2a1cre but not Piezo2^Col2a1Cre mice, scale bars: 1 mm. d µCT-based quantification of subchondral bone volume fraction (BV/TV) and trabecular number (Tb.N). e µCT-based quantification of calcified meniscus and synovium assessed in the lateral and medial joint compartment of non-operated and ACLT control, Piezo1^Col2a1cre, and Piezo2^Col2a1Cre mice