Fig. 6.

Osteophyte formation and maturation are markedly reduced in Piezo1^Col2a1cre mice. a Representative µCT images of knee joints after ACLT from control and Piezo1^Col2a1Cre mice showing smaller osteophytes in Piezo1^Col2a1Cre mice, scale bar: 1 mm. b µCT-based quantification of the bone volume (BV) of osteophytes and c osteophyte total volume (TV). d Representative, Safranin-O stained image of tibial osteophytes highlighting the immature cartilaginous osteophyte composition in Piezo1^Col2a1Cre mice, scale bar: 100 µm. e Quantification of osteophyte size and f osteophyte maturity. n ≥ 8. Statistical analysis was conducted with one-way ANOVA (Tukey). *P < 0.05, **P < 0.01, ***P < 0.001