FIG. 3.

Binding of TFII-I to the c-fos SIE. (A) TFII-I, prepared as described for Fig. 2A, was incubated with ³²P-labeled c-fos SIE probe and processed for EMSA as described in Materials and Methods. Anti-TFII-I antibody (α-TFII-I) or the indicated unlabelled oligonucleotide competitor DNAs were preincubated with affinity-purified TFII-I for 15 min at room temperature where indicated. (B) Affinity-purified recombinant TFII-I was incubated with ³²P-labeled c-fos basal probe (−60 to +18) and processed for EMSA as described in Materials and Methods. Anti-TFII-I antibody or the indicated unlabelled oligonucleotide competitor DNAs were preincubated with the extracts for 15 min at room temperature where indicated.