FIG. 7.

TFII-I forms in vivo complexes with SRF, STAT1, and STAT3. SRF (A), Elk-1 (B), STAT1 (C), and STAT3 (D) expression plasmids were cotransfected into COS-1 cells with pEBG (lanes 1 and 3) and pEBG-TFII-I (lanes 2 and 4) expression plasmids. Transfected COS-1 cells were lysed and subjected to GST pulldown assay as described in Materials and Methods. Ten micrograms of each total lysate (lanes 1 and 2) and glutathione (GSH)-Sepharose bead-bound proteins (lanes 3 and 4) were fractionated by SDS-PAGE (12% polyacrylamide). Proteins associating with GST or GST–TFII-I were detected by Western blot analyses with anti-SRF (A), anti-Elk-1 (B), anti-STAT1 (C), or anti-STAT3 antibody (Ab).