FIG. 2.

Endo-H sensitivities of the different RET forms. Cos cells were transfected with the RET constructs indicated above the lanes and harvested 48 h later. Protein extracts were prepared and treated with Endo-H or were left untreated. The products were analyzed on an SDS–7.5% PAGE gel and immunostained with anti-RET antibodies. The migration of the fully glycosylated and partially glycosylated proteins and of the digestion product is shown on the left.