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. 2024 Jan 23;300(3):105684. doi: 10.1016/j.jbc.2024.105684

Figure 4.

Figure 4

EEF1A1 inhibition increases neutral lipid accumulation in HepG2 cells.A, triglyceride (TG) and (B) cholesteryl ester (CE) masses measured in HepG2 cells pretreated with DMSO or didemnin B (DB) (80 nM) in basal media for 24 h, followed by treatment with media containing BSA alone or 1 mM palmitate plus oleate (2:3 ratio, PA/OA) with DMSO or DB for 6 h, n = 5. C, total neutral lipid mass, calculated as the sum of TG and CE, n = 5. D, confocal micrographs of HepG2 cells stained with Oil Red O (neutral lipid, red) and DAPI (nuclei, blue) after pretreatment with DMSO or DB (80 nM) in basal media for 24 h, followed by treatment with media containing PA/OA with DMSO or DB for 6 h. The scale bar represents 10 μm. E, lipid droplet (LD) size distributions, generated using particle analysis in ImageJ, n = 4. F, images from D colorized based on the LD size bins and corresponding colors from the x-axis in E. The scale bar represents 10 μm. G, median LD size, (H) LD number, and (I) total LD area determined from data in E. Data are means ± SD. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. CHO, Chinese hamster ovary; EEF1A, eukaryotic elongation factor 1A.