Figure 2.

Confirmation of MUC2 extraction from feces. A, schematic of human MUC2 extraction and purification. B, periodic Acid-Schiff staining of mucin-purified from feces. PGM = commercial crude porcine gastric mucin as a positive control for high molecular weight mucin. C, Western blot of MUC2 (rabbit polyclonal IgG, used in Fig. 1) purified from feces and separated by SDS-UAgPAGE pre- and post-LPS removal. Arrow indicates high molecular weight MUC2. White space between lanes indicates these lanes were not adjacent on original gel. PCM, Porcine colonic mucin; Hu, human; Mouse, m . D, Heatmap of spectral counts of proteomic profile from extracted MUC2/Muc2 from human and mouse. E. PCoA biplot of proteins found on human MUC2.