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. 2024 Jan 24;300(3):105675. doi: 10.1016/j.jbc.2024.105675

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© 2024 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Analysis of MUC2 glycosylation on fecal sections in situ. A, schematic of glycan structures recognized by the lectins used in this figure to assist in interpretation. B, Tiled cross-section of healthy human feces dual stained with Wheat Germ Agglutinin (WGA) and Aleuria aurantia lectin (AAL). C and D, high-mag imaging human mucus stained for Maackia ammurensis (MAL)-II and Sambucus nigra (SNA)-1 (B), and Lycopersicon esculentum (Tomato) lectin (LEL) and Ulex europaeus-1 (UEA-1) (C). E, Lectin blotting of fecal MUC2. Lanes that are close together represent samples run on the same gel and transferred to the same membrane, but separated for specific blots. White space indicates lanes were not adjacent. Bar on the right of blot = 250 kDa maker. F, dual stain for MUC2 and MALII to analyze MALII distribution in human versus mouse fecal sections. Results are representative of five individual fecal sections.