Figure 4.

Comparative glycomic analysis of human fecal MUC2 with other sources. A, representative extracted ion chromatograms (EICs) of all sialylated O-glycans detected after HPLC-MS analysis of a single human sample. Each peak represents a sialylated glycan. Not all peaks are annotated for simplicity. Annotations refer to monosaccharide compositions (HexNAc = N-acetylhexosamine; Hex = Hexose; Fuc = Fucose, Neu5Ac = Sialic Acid; subscripts = number of monosaccharides. The peak areas for all glycans were normalized to the totals for the remaining comparisons in Panels b through e. B, Venn diagram showing overlap of common glycan structures identified from ammonia-catalyzed beta-elimination of O-linked oligosaccharides from indicated sources. Total unique and common glycans are indicated as total number with % of total analyzed in parentheses. C, pie chart of overall acidic versus neutral glycans from human fecal, mouse fecal and porcine colonic MUC2. D, waffle plot showing relative abundance of specific classes of glycans indicated by color. E, Heatmap of log2-transformed relative abundances of individual glycans found in both human and porcine colon mucin sample, with each row representing a unique structure. HF-MUC2, human fecal MUC2; MF-Muc2, mouse fecal Muc2; PC-MUC2, porcine colonic MUC2.