Table 2.

Optimization steps for Capto^TM Core 700 chromatography.

Step	Parameter
System	ÄKTA™ purifier (Cytiva, Uppsala, Sweden)
Column	XK16/40 (Cytiva, Uppsala, Sweden), column height = 16 cm, CaptoTM Core 700
Sample	H5N1 HPAIV (NIBSC, Hertfordshire, UK) ultrafiltration concentrate
Sample load	100% BV, 100 cm/h
Running buffer	150 mmol/L NaCl + 20 mmol/L PB, pH 7.5, 100 cm/h
Wash	150 mmol/L NaCl + 20 mmol/L PB, pH 7.5, 100 cm/h
Cleaning in place (CIP)	0.5 M NaOH, 60 cm/h