Table 2.

Cytofix/cytoperm solution inactivates attenuated and wild-type RVFV. Triplicate samples of SIM A9 cells were infected with RVFV MP-12 at an MOI of 8 (for an initial protocol development trial) or RVFV ZH501 at an MOI of 10 (for 3 subsequent validation trials) and harvested after 24 h in culture. Each sample was split into 2 aliquots, with one half being plated directly onto a confluent monolayer of Vero cells for detection of live virus (Inactivated − spike) while the other half was spiked with live virus, then plated on a Vero cell monolayer (Inactivated + spike). A negative control well was treated with PBS alone. No plaques were detected in the neg control or inactivated wells (nd). Total destruction of the monolayer was observed in all wells containing spiked sample B. nd = none detected. TNTC = too numerous to count. TDOM = total destruction of monolayer.

Virus	MP-12			ZH501
Replicate #	1	2	3	1	2	3
Negative control	nd	–	–	nd	-	-
Inactivated − spike	nd	nd	nd	nd	nd	nd
Inactivated + spike	TNTC	TNTC	TNTC	TDOM	TDOM	TDOM