FIG. 2.
The mutations C500Y and L501P in the GR DBD eliminate binding to GST–Oct-1 POU in vitro. (A) Schematic summary of the DNA binding domain of the rat GR DBD showing the position of the zinc-coordinating cysteines and the two α-helices that contact DNA. (B) The in vitro-translated GRWT DBD (aa 407 to 568) (lanes 8 and 19) and specific point mutation-containing peptides (all of which were in the aa 407 to 556 GR backbone except G504R, which was in the aa 407 to 523 backbone) (lanes 2 to 11) were tested for binding to GST–Oct-1 POU (lanes 2 to 11) in comparison to that of X616 (lanes 1 and 12), as described for Fig. 1.