FIG. 5.
Two-hybrid analysis of the binding of the GR DBD to the Oct-1 POU domain in CHO cells as reflected by the activation of pG5E1bCAT transcription. Gal-GRWT, Gal-GRL501P, or the Gal4 DBD (GalO) were coexpressed with the VP16 activation domain alone (left) or tethered to the POU domain of Oct-1 (right). The means and standard errors of the means of results of five independent transfections are shown. Western blot analysis indicated that GalO, Gal-GRWT, and Gal-GRL501P were expressed at the same level (73). Similar results were also obtained with HeLa cells (73).