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. 2024 Feb 9;17(2):230. doi: 10.3390/ph17020230

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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SAMA shows apoptosis in oral cancer cells. (A) Annexin V/7-aminoactinmycin D (7AAD) assay of SAMA. Cells were treated with 0.1% DMSO (untreated control) and SAMA (0, 15, and 25 μg/mL) for 24 h. The proportion of annexin V (+)/7AAD (±) (%) was regarded as apoptosis (+) (%). (B) Annexin V/7AAD assay of NAC/SAMA. NAC/SAMA represents the pre-treatment of 10 mM NAC for 1 h and the post-treatment of 25 μg/mL SAMA for 0, 12, and 24 h. Data are shown as means ± SD (triplicate). An absence of overlapping annotations (lowercase notes) between treatments indicated a significant difference (p < 0.05).

SAMA shows apoptosis in oral cancer cells. (A) Annexin V/7-aminoactinmycin D (7AAD) assay of SAMA. Cells were treated with 0.1% DMSO (untreated control) and SAMA (0, 15, and 25 μg/mL) for 24 h. The proportion of annexin V (+)/7AAD (±) (%) was regarded as apoptosis (+) (%). (B) Annexin V/7AAD assay of NAC/SAMA. NAC/SAMA represents the pre-treatment of 10 mM NAC for 1 h and the post-treatment of 25 μg/mL SAMA for 0, 12, and 24 h. Data are shown as means ± SD (triplicate). An absence of overlapping annotations (lowercase notes) between treatments indicated a significant difference (p < 0.05).