Figure 3.

Visualization of treated and untreated EV samples obtained by low-voltage scanning electron microscopy. (a) EV sample sequentially treated with Triton X 100, proteinase K, and RNAse. (b) Control slide with DPBS. (c) EV sample treated with proteinase K and RNAase. Enlarged field marked with dotted rectangle. (d) Enlarged single EV, demonstrating absence of protein corona on the outer surface. (e) Untreated EV sample. Enlarged field marked with dotted rectangle. (f) Enlarged single EV, demonstrating presence of protein corona on the outer surface.