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. 2024 Jan 24;12(2):248. doi: 10.3390/microorganisms12020248

Figure 2.

Figure 2

Cell viability of HCAECs stimulated with P. gingivalis W83 for 24 h at M OI 1 and MOI 100. (A–F) Green fluorescence indicates the live bacteria and red fluorescence indicates the dead bacteria. (A) Bacterial inoculum adjusted for stimulation of HCAECs; (B) P. gingivalis W83 was treated with 0.2% chlorhexidine as a control to create dead cells; (C) HCAECs in the 2D culture were stimulated with P. gingivalis W83 at MOI 1; (D) HCAECs in the 2D culture were stimulated with P. gingivalis W83 at MOI 100; (E) HCAECs in the 3D culture were stimulated with P. gingivalis at MOI 1; (F) HCAECs in the 3D culture were stimulated with P. gingivalis W83 at MOI 100; (G) The cell viability of HCAECs stimulated with P. gingivalis W83 at MOI 1 and MOI 100 in 2D and 3D cell cultures. Control: control without stimulus. MOI 100: a multiplicity of infection for P. gingivalis/HCAECs of 100:1. MOI 1: a multiplicity of infection for P. gingivalis/HCAECs of 1:1. p < 0.05 compared with the control, ns: Not significant. The data are represented by the median of each group of three independent experiments with eight replicates.