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. 2024 Feb 18;16(4):563. doi: 10.3390/nu16040563

Figure 3.

Figure 3

Analysis of the effects of Nrf2 inhibition on the suppression of LPS-induced inflammation by tuber extracts: (A) Relative production level of IL-6 at 24 h after LPS stimulation with/without tuber extracts and ML385; (B) Relative expression of pro-inflammatory genes (IL-6, Tnf, iNOS(Nos2)) at 24 h after LPS stimulation with/without tuber extracts and ML385; (C) Western blot of nuclear and cytoplasmic fractions at 180 min after LPS stimulation in the presence of tuber extracts and ML385; (D–F) Relative quantification of nuclear NF-κB, Nrf2 and cytoplasmic HO-1 under each condition: (A,B,D–F) mean ± SEM, one-way ANOVA with Tukey’s post-hoc test, *: p < 0.05, ****: p < 0.0001, Distinct symbols (a, b, c, d) are used to denote significant differences between groups: LPS, lipopolysaccharide; Ext, extract.