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. 2024 Feb 8;13(2):154. doi: 10.3390/pathogens13020154

Figure 3.

Figure 3

Deletion screening on samples collected on day of euthanasia. (A) PCR with primers covering nt 6188-17145. L1: Genomic Ladder, L2: GeneRuler 1kb plus, Inoc: Inoculum, E1: Pig 1 EDTA-blood, E2: Pig 2 EDTA-blood, E4: Pig 4 EDTA-blood, E7: Pig 7 EDTA-blood, E8: Pig 8 EDTA-blood, E9: Pig 9 EDTA-blood, E10: Pig 10 EDTA-blood, E11: Pig 11 EDTA-blood, E12: Pig 12 EDTA-blood. (B) PCR with primers covering nt 6188-17145. L1: Genomic Ladder, L2: GeneRuler 1kb plus, E8: Pig 8 EDTA-blood, B8: Pig 8 bone-marrow, SP8: Pig 8 spleen, S8: Pig 8 serum. (C) PCR with primers covering nt 6708-7668. L1: D5000 Ladder, L2: GeneRuler 1kb plus, Inoc: Inoculum, E1: Pig 1 EDTA-blood, E2: Pig 2 EDTA-blood, E4: Pig 4 EDTA-blood, E7: Pig 7 EDTA-blood, E8: Pig 8 EDTA-blood, E9: Pig 9 EDTA-blood, E10: Pig 10 EDTA-blood, E11: Pig 11 EDTA-blood, E12: Pig 12 EDTA-blood. (D) PCR with primers covering nt 6708-7668. L1: D5000 Ladder, L2: GeneRuler 1kb plus, E8: Pig 8 EDTA-blood, B8: Pig 8 bone-marrow, SP8: Pig 8 spleen, S8: Pig 8 serum. Green and purple bands indicate lower and upper molecular weight markers, respectively, whereas arrows indicate bands detected by the TapeStation Analysis software.