Table 1.

Primer overview.

Name	Fragment	Sequence (5′-3′)
ASFV-300-F α	01_F	AGGTGGTTTGGCCGTATTCT
ASFV-11927-R α	01_R	ATGCGTAGGCCTCCTGAAAG
ASFV-10819-F α	02_F	ATAGGAGCGGCTTGAAGGAC
ASFV-22803-R α	02_R	TGCGGCAACATATGTCCAAAC
ASFV-22129-F α	03_F	CAAAGATGCCGTACCTCCGA
ASFV-33909-R α	03_R	TTTACGGCTTGGGTCAGGAC
ASFV-33237-F α	04_F	GCTCCCTTCAACGCATAGGA
ASFV-44936-R α	04_R	TGCGGGTCTTGGATTAAAGGA
ASFV-43757-F α	05a_F	TGGACCCAAAAAGGGTGGTC
ASFV-56602-R α	05a_R	GCGGCATTGAAAAACACCCT
ASFV-55750-F α	05b_F	TGAGCTGTTCCCAGGATTCG
ASFV-68434-R α	05b_R	AGCGCGCGTATTTATCAACG
ASFV-67660-F β	06_F	GGACTGCGACACGATCACAGAGTC
ASFV-78076-R β	06_R	GTCCTACGACACCATGCGAACCAAG
ASFV-77808-F β	07_F	CTATATTGGCAAACTGTTTCACGTC
ASFV-87981-R θ	07_R	CAATCACAACGGTTCTCCTGTTAAG
ASFV-87000-F α	08_F	GCATAATCAATGGCAATCCCCC
ASFV-97970-R α	08_R	TGGCCTTAATCATTACAGCGGT
ASFV-97345-F β	09_F	GTTACGTAGATCACTGAGTTGCAATC
ASFV-107683-R β	09_R	GGCGCCCTCCTATACGATGAG
ASFV-107531-F β	10_F	GTGTCCTCCATCGGATATACTATAC
ASFV-117620-R θ	10_R	AGTGTGCTGACCTATATCACGGAAC
ASFV-117410-F β	11_F	CATTTCTGAACTGCGAGAGTTCTAG
ASFV-127433-R β	11_R	TCGCTGTGCGTAATTTATCCCAATC
ASFV-126429-F α	12_F	AACACCTAACCTCGTCGTGC
ASFV-137952-R α	12_R	ACAGGTAAGGTCCGACTCGT
ASFV-137097-F β	14_F	GAGAACAGGTCTTAGAATTACTTCATG
ASFV-147178-R β	14_R	ACGCATCCGAAGGTGTTACAAGGAC
ASFV-146744-F θ	15_F	CTCTGAATGCGCAGAGCATCTTAC
ASFV-156426-R β	15_R	GAACATGGGAATACGTGTGTCCAG
ASFV-155654-F α	17_F	AGGAACTGGACATGCAAGCAG
ASFV-166805-R α	17_R	ATGAGCTCGCCCACATAACC
ASFV-166089-F α	18_F	TATTGCCCGAGCCTCTGTATTC
ASFV-177600-R α	18_R	GGGGGAATCAACTCTCGCTTAA
ASFV-6188-F α	del-PCR_F	GCTTCTAACTCTCTGTACAACA
ASFV-17145-R α	del-PCR_R	CGGCATATCATAAGTAGGTTGGT
ASFV-6708-F α	noDel-PCR_F	AAGTGGCTGCTCGTCAACAA
ASFV-7668-R α	noDel-PCR_R	AGCCGTAGCAATGTTGGTGA

^α Designed in this study; ^β Designed by Portugal et al. [34]; ^θ Modified from Portugal et al. [34]