FIG. 1.

Yeast one-hybrid assay of HOX11 transcriptional activation domains. GAL4-DBD-HOX11 fusions are represented schematically on the left. The position of the homeodomain is indicated by the dashed vertical lines, and mutants lacking the third helix of the homeodomain (amino acids 242 to 251) are marked by the symbol ΔH3. The ability of each fusion to activate the lacZ reporter gene, as assessed by the presence of a blue color in the slot blot β-galactosidase assay, is indicated on the right. Apparent differences in the intensity of blue coloration are ignored because although expression of the GAL4-DBD alone and fusions Y1 to Y11 was confirmed by Western blot analysis with a monoclonal GAL4-DBD antiserum, the levels of protein expression varied (data not shown).