Table 5.
HPLC chromatography gradient.
| Time | Solvent B |
|---|---|
| 0 min | 2% |
| 5 min | 2% |
| 10 min | 8% |
| 60 min | 31.2% |
| 66 min | 50% |
| 69 min | 100% |
| 72 min | 100% |
| 75 min | 2% |
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