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FACS results obtained by testing F1 (a) and F2 (b) liposomes. Non-activated cells are represented by blue bars, while pink bars represent TNF-α-activated cells. 1: negative controls (plain cells); 2: positive controls (cells receiving anti-VCAM-1 antibody); 3: cells treated with only NeutrAvidinTM followed by F1 or F2 liposomes; 4: cells treated with the complete three-step pretargeting protocol: first NAMP, followed by NeutrAvidinTM and F1 or F2 liposomes.
