FIG. 3.
Induction of p105HEF1 and p115HEF1 following serum stimulation in MCF-7 cells. MCF-7 cells were brought to quiescence by starvation for serum (St.), then the medium was changed to DMEM–10% calf serum, and lysates were made at the times indicated after medium addition (15 or 30 min and 1, 2, 4, 6, or 24 h). Crude cell lysates were resolved by SDS-PAGE, and HEF1 species were visualized by α-HEF1; cross-reactive p95 confirms the equal loads of lanes. Numbers at right and left indicate molecular mass in kilodaltons.