Table 1.

Fractions (F1–F11) from flash chromatography with concentrations of T-2 toxin, HT-2 toxin, and HT-2-glucoside, as detected and quantified by LC-MS/MS; <LOD, limit of detection; * fraction used for NMR analysis (with highest amount of HT-2-glucoside).

Fraction Nr.	Elution Time (min.)	Volume (mL)	Metabolites Detected (µg/L)
			HT-2- Glucoside	HT-2 Toxin	T-2 Toxin
1	3–8	75	<LOD	<LOD	<LOD
2	9–12	85	<LOD	<LOD	<LOD
3	14–15	86	<LOD	<LOD	1
4 *	20–24	61	8305	95	9
5	24–27	55	136	127,650	44
6	28–30	56	<LOD	1482	191,500
7	31–33	39	<LOD	1114	137,600
8	34	32	<LOD	287	122,700
9	36–39	59	<LOD	155	12,800
10	40	92	<LOD	79	4554
11	45–51	156	<LOD	75	3307