Fig. 4 –

Multiple dosing of FI elicits greater reduction in proliferation. (A) N-terminal GPC3 protein expression is reduced in HepG2 cells with control (C) and furin inhibitor (FI) shown for each treatment regimen at 24 h and 48 h. Full-length GPC3 and furin protein expression was not significantly changed. (B) Reduced proliferation was observed 48 h after treatment of HepG2 cells with FI every 8 h (q8h) and every 24 h (q24h), but not at 24 h. (C) Furin protein expression in SKHep1 cells after treatment with FI showed no significant change between control and treated cells. (D) SKHep1 proliferation at 24 h and 48 h in SKHep1 cells after treatment with 100 μM furin inhibitor every 8 h (q8h) and 24 h (q24h) showed no significant change. At the 48 h timepoint, q8h dosing resulted in cell death preventing analysis.