Skip to main content
. 2019 Jan 29;40(9):1110–1120. doi: 10.1093/carcin/bgz015

Figure 3.

Figure 3.

Inhibition of autophagy does not impact WFA-mediated inhibition of cell survival and apoptosis induction in breast cancer cells. (A) MCF7 and MDA-MB-231 cells were treated with 5 µM WFA alone or in combination with 200 nM Baf, 25 μM CQ and 4 mM 3-MA as indicated and subjected to MTT assay. *P< 0.001, compared with control. (B) Breast cancer cells were treated with 5 µM WFA alone or in combination with 25 μM CQ and 4 mM 3-MA as indicated and subjected to DNA fragmentation assay. *P< 0.01, compared with control. (C) MCF7 and MDA-MB-231 cells were treated with 5 µM WFA and 4 mM 3-MA and total cell lysates were immunoblotted for cleaved PARP1 (cPARP1, 89 kDa), PARP1 (116 kDa) and ACTB (45 kDa) as indicated. (D) CRISPR/Cas9 was used to knockout BECN1 and ATG7 in MCF7 cells and total cell lysates were immunoblotted for BECN1 (60 kDa) and ATG7 (78 kDa). ACTB (45 kDa) was used as loading control. (E) Vector-control, BECN1-KO and ATG7-KO MCF7 cells were treated with 5 µM WFA for indicated time intervals and total cell lysates were immunoblotted for cleaved-PARP1 (89 kDa) and total-PARP1 (116 kDa) expression levels. ACTB (45 kDa) was used as loading control. (F) Clonogenicity of control, BECN1-KO and ATG7-KO MCF7 cells treated with 5 µM WFA as indicated. (G) Cell viability of control, BECN1-KO and ATG7-KO MCF7 cells treated with 5 µM WFA was examined using MTT assay. Representative pictures of cells are shown.