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Mutant transcript analysis of e14.5 GnaqRQfl/wt and GnaqRQfl/wt;β-actin-Cre+ embryos. SNaPshot analysis was employed to assay the mutant allele transcript levels from whole embryo tissue. All of the GnaqRQfl/wt embryos had less than 5% mutant allele expression. Conversely, the GnaqRQfl/wt;β-actin-Cre + embryos all had mutant allele expression levels of 100%.
