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. Author manuscript; available in PMC: 2024 Apr 21.
Published in final edited form as: ACS Chem Biol. 2023 Mar 27;18(4):837–847. doi: 10.1021/acschembio.2c00898

Table 1.

Binding constant of UIMC1, AN13A and USP37 UIMs interacting with diUb and branched triUb determined by BLI.

Probe UIMC176-128
Kd (μM)		 AN13A479-594
Kd (μM)		 USP37700-851
Kd (μM)
K11-diUb 2.92 ± 0.46 1.28 ± 0.21 8.89 ± 0.83
K63-diUb 2.17 ± 0.23 0.25 ± 0.03 4.97 ± 1.07
K11/K48-triUb-(1,0) --- 7.83 ± 1.89 ---
K63/K48-triUb-(1,0) 2.43 ± 0.62 0.74 ± 0.69 2.71 ± 0.52
K11/K63-triUb-(1,0) 1.04 ± 0.10 0.43 ± 0.03 3.36 ± 0.89

--- indicates no significant binding observed.