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AML/ETO repression of MDR-1 requires DNA binding and fusion with ETO sequences. C33A cells were transfected with 5 μg of MDR-1 CAT and 1 μg of Rous sarcoma virus SEAP plasmids and 2 μg of the indicated CMV5 AML/ETO (A/E) fusion proteins. CAT activity was quantitated with a Molecular Dynamics PhosphorImager and normalized relative to SEAP activity.
