FIG. 6.

The HHR motif is required for AML/ETO/ETO heterodimers and AML/ETO homodimers. (A) Cos cells were transfected with 2 μg of the indicated CMV5 AML/ETO (A/E) and CMV5 ETO plasmids, and 48 h posttransfection cells were labeled with [³⁵S]methionine as described in Materials and Methods. Equal trichloroacetic acid-precipitable counts were immunoprecipitated with the antibodies listed on the bottom of the diagram, as described in Materials and Methods. “Boil” indicates that the sample was heated to 100°C for 1 min prior to the immunoprecipitation. Protein A-Sepharose was used to collect immunocomplexes, which were then analyzed by SDS-PAGE in an 8% gel. The gel was then dried and subjected to autoradiography. (B) Cos cells were transfected with HA-AML/ETO (HA A/E) and the indicated AML/ETO deletion constructs and were labeled with [³⁵S]methionine, immunoprecipitated, and analyzed by SDS-PAGE as described for panel A.