FIG. 7.

High expression levels of ETO can inhibit AML/ETO function. (A) Western blot analysis of ETO expression in HEL and C33A cells. Cell lysates (150 μg) from HEL or C33A cells were fractionated by SDS-PAGE in an 8% gel, transferred to nitrocellulose, and blotted with ETO antiserum as described in Materials and Methods. (B) C33A cells were transfected with 2 μg of MDR-1, 0.1 μg of CMV5 AML/ETO, 100 ng of CMV β-galactosidase, and increasing amounts of CMV5 ETO as indicated. CAT assays were quantitated and normalized to β-galactosidase activity.