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. 2024 Feb 24;15:1685. doi: 10.1038/s41467-024-45995-5

Fig. 2. Loading of ECM1 protein into sEVs is associated with integrin-β2.

Fig. 2

a Integrin-β2 protein expressions in the circulating sEVs of high-fat diet-induced obesity mice (D-sEVs) and control diet mice (C- sEVs). b Single cell specific transcriptome profiles showing ECM1 expressions in different cell types. c Single cell sequencing analysis showing ECM1 expressions in peripheral blood mononuclear cells (PBMC) including macrophages. d Single cell type specificity showing integrin-β2 expressions in PBMC including macrophages. e Integrin-β2 protein levels in the integrin β2-knockdown and control RAW264.7 cells. f ECM1 and (g) ICAM1 and GPX3 protein levels in the sEVs released by integrin-β2-knockdown and control RAW264.7 cells. Integrin-β2 protein levels (h) in integrin-β2-overexpressed and control RAW264.7 cells and (i) in the sEVs released by these cells. Shown is the mean ± SD; two-sided unpaired t-test for (a, e to i); n = 3 independent experiments; p values are indicated in graphs. si-β2 integrin-β2-knockdown cells, NC control cells, ICAM-1 intercellular cell adhesion molecule-1, GPX3 glutathione peroxidase 3, ECM1 extracellular matrix protein 1, CD control diet, DIO high-fat diet-induced obesity. Source data are provided in Source Data file.