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. 2024 Feb 24;15(2):170. doi: 10.1038/s41419-024-06544-6

Fig. 4. meR206-PGK1 inhibits the TCA cycle by promoting mitochondria localized PGK1 mediated PDHK1 phosphorylation.

Fig. 4

A–D Oxygen consumption rates (OCAR) were analyzed in HCT116 cells in which PRMT1were stable overexpressed (A), knocked down (B) when HCT116 cells were treated with GSK3368715 (C), and in stable overexpressed wild and mutant Flag-PGK1 (WT, R206K) HCT116 cells (D) by Seahorse Bioscience Extracellular Flux Analyzer. The working concentration of the drugs showed in A–D was Oligomycin (10 μM), FCCP (1 μM), and Antimycin A/Rotenone (50 μM). E–L PDHK1, PDHA1 and p-PDHK1, p-PDHA1 phosphorylation levels were detected in PRMT1 stable overexpressed (E, F), knocked-down (G, H) HCT116 cells and when HCT116 cells were treated with GSK3368715 (3.1 nM, 48 hours) (I, J), and in stable overexpressed wild and mutant Flag-PGK1 (WT, R206K) HCT116 cells (K, L).