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. 2024 Feb 24;15:1697. doi: 10.1038/s41467-024-45969-7

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a CBE6 variants or existing cytosine base editors, all using SpCas9 nickase domains in the BE4max architecture, were transfected into HEK293T cells with guide RNAs targeting three protospacers. Data are presented as mean values ± SD. Dots represent individual values from n = 3 independent biological replicates. PAM sequences are underlined. HEK293T site 2 is abbreviated HEK2, and HEK293T site 4 is abbreviated HEK4. b CBE6 variants along with existing cytosine base editors using eNme2-C Cas9 nickases in the BE4max architecture were transfected into HEK293T cells with guide RNAs targeting three protospacers. Data are presented as mean values ± SD. Dots represent individual values from n = 3 independent biological replicates. PAM sequences are underlined. Full data for C•G-to-T•A and A•T-to-G•C activity are in Supplementary Figs. 12 and 13. Source data are provided as a Source Data file.