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. 2024 Feb 24;7:227. doi: 10.1038/s42003-024-05898-z

Fig. 3. Impaired BMP signaling perturbs decidualization in the endometrium of individuals with endometriosis.

Fig. 3

A, B Lysates from endometrial stromal cells of individuals without (A, “normal”) with endometriosis (B) after 2, 4, 6, or 8 days of EPC treatment were probed with antibodies to detect phosphorylated SMAD1/5 (pSMAD1/5), total SMAD1, total SMAD5, or GAPDH expression. C Densitometric analysis of pSMAD1/5 in the EPC-treated stromal cells from individuals without (n = 5) or with endometriosis (n = 4). The different symbols represent individual patient trajectories per sample. One way ANOVA with a Tukey’s posttest. D, E Quantitative reverse transcriptase PCR (qRT-PCR) was used to determine the expression of BMP2 and IGFBP1 in the endometrial stromal cells from individuals without (C, n = 4) or with endometriosis (D, n = 4). The different symbols represent individual patient trajectories per sample. Data in (D, E) were log transformed and analyzed using a 2-Way ANOVA with a Tukey’s multiple comparison posttest. F, G Time course analysis from the RNAseq analysis comparing the increasing gene expression patterns of ID1 and ID3 in normal and decreasing gene expression pattern in endometriosis stromal cells. EPC, 35 nM estradiol, 1 µM medroxyprogesterone acetate, 50 µM cAMP.