Induction of apoptotic morphology by posttranslationally modified LEI. Purified nuclei from BHK cells were incubated for 0, 2, or 6 h in the presence of LEI produced by E. coli without further treatment (LEI), LEI treated overnight at pH 2.0 (LEI pH 2), or PBS. (A) Neutral agarose gel of BHK purified nuclei after incubation for 2 or 6 h in the presence of the different proteins. (B) The purified nuclei were treated as above, stained with DAPI, and observed with a fluorescence microscope.