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Fluorescence spectrum of ICG and HSA at different concentrations and ratios. (A) ICG concentration of 5×10−4 M; (B) ICG concentration of 5×10−5 M; (C) ICG concentration of 5×10−6 M; (D) ICG concentration of 5×10−7 M; (E) ICG concentration of 5×10−8 M; (F) ICG concentration of 5×10−9 M. ICG, indocyanine green; HSA, human serum albumin.
